. 2020 Jun 4;8(2):275. doi: 10.3390/vaccines8020275

Table 2.

Enzyme-linked immunospot (ELISPOT) analysis of activated mice splenocytes.

Immunisation Groups	Spot Count ¹
Buffer only	0.67 ± 0.58 ^a
Buffer and alum	0.00 ± 0.00 ^a
Sf9-produced Nc-aD VLPs	26.67 ± 0.57 ^b
E. coli-produced Nc-aD VLPs	6.50 ± 0.45 ^c
Engerix B	11.30 + 0.71 ^d
Sf9-produced Nc VLPs	0.33 ± 0.58 ^a
E. coli-produced Nc VLPs	0.33 ± 0.58 ^a

¹ Spot counts represent ASCs per 1.25 × 10⁵ splenocytes. The letters (a, b, c, d) represent the statistical significance of the measurements. Spot count values which are marked by different letters are significantly different (p < 0.001), and those marked by the same letters are not significantly different.