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. 2020 Jun 25;21(12):4514. doi: 10.3390/ijms21124514

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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hnRNP H1 upregulated sphingosine-1-phosphate lyase 1 (SGPL1) mRNA and promoted colorectal cancer progression. (A) The SRB assay (n = 5) showed that SGPL1 downregulation resulted in the strongest inhibition of cell growth in HCT116 cells. (B) RNA immunoprecipitation combined with RT-PCR (n = 3) confirmed the direct binding of hnRNP H1 and SGPL1 mRNA. (C) RT-PCR (n = 3) and western blotting (n = 3) showed the decrease in SGPL1 mRNA and protein in hnRNP H1-downregulated HCT116 cells. (D) RT-PCR (n = 3) and western blotting (n = 3) showed that the downregulation of SGPL1 mRNA and protein was not observed in hnRNP H1-downregulated HECE-1CT cells. (E) The SRB assay (n = 5) showed that an additional tumor-suppressive effect was not observed by SGPL1 downregulation. (F) The SRB assay (n = 5) showed that cell growth was significantly increased in the hnRNP H1-downregulated HCT116 cells with S1P in a concentration-dependent manner compared to those without sphingosine-1-phosphate (S1P). The error bars show the SD. * p < 0.05 by Student’s t-test.