Figure 6.

siRNA of hnRNP H1 or SGPL1 upregulated p53 phosphorylation and inhibited cell growth. (A) Western blotting (n = 3) revealed that phosphorylated p53 was significantly increased in hnRNP H1- and SGPL1-downregulated cells, whereas p53 did not change. (B) The SRB assay (n = 5) revealed that the tumor-suppressive effect induced by downregulating hnRNP H1 or SGPL1 after 72 h in cultured HCT116 cells was diminished by treatment with the p53 inhibitor, pifithrin-μ. (C) The SRB assay (n = 5) revealed that the tumor-suppressive effect induced by downregulating hnRNP H1 or SGPL1 was less influential in HCT116 p53^−/− cells than in HCT116 parental cells (C). The error bars show the SD. * p < 0.05 by Student’s t-test.