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. 2020 May 27;8(2):251. doi: 10.3390/vaccines8020251

Figure 3.

Figure 3

Monocyte subsets in the lymph nodes (LNs) of immunized mice: C57BL/6 mice were immunized i.m. with MERS-RBD-Fd with or without alum and rASP-1 alone or together using different combinations and/or formulations (Table 1 and X-axis legend). The draining LNs were harvested 7 days post-2nd immunization and the number of cells per LN were analyzed. (A) Representative flow cytometry plot of CD40+ monocytes (upper panel) and CCR7 + monocytes (lower panel in each immunization group. (B) The number of CD40+ monocytes, and (C) CCR7+ monocytes per LN were analyzed. The experiment was done once, and the data presented is from left and right draining LNs of 5 mice per group: mean and standard error. “+” indicates the presence and “−“ indicates the absence of the protein or adjuvants in the formulation. Statistics was performed using one-way ANOVA with Tukey’s multiple comparison. p < 0.05: *, p < 0.001: ***.