Table 1.

Immunization of mice using different combinations and/or formulations of the vaccines and the site of injection: C57BL/6 mice were immunized intramuscularly (i.m.) with MERS-RBD-Fd (RBD) formulated with or without alum and/or rASP-1 alone or together in different combinations and/or formulations. Mice were immunized twice, 3 weeks apart according to the various G1–G6 experimental groups either at the front (A: 50 µL of inoculum) and/or the back (B: 50 µL of inoculum) of the caudal thigh muscle in each hind leg.

Groups	Injection Site A (50 µL Front Caudal Thigh Muscle)	Injection Site B (50 µL Back Caudal Thigh Muscle)
1	PBS	PBS
2	RBD	PBS
3	RBD + rASP-1	PBS
4	RBD + alum	PBS
5	RBD + rASP-1 + alum	PBS
6	RBD + alum	rASP-1