Figure 2.

Bright-field photomicrograph of olfactory marker protein (OMP) staining in the rat testes. (A) Control tissue containing seminiferous tubules (ST) and interstitial tissue (IT) is stained by cresyl violet but there is no immunostaining when the OMP antibody is left out. Leydig-like cells are shown on the right at higher magnification and are void of OMP reaction product. (B) Tissue stained by OMP antibodies reveal Leydig-like cells express OMP (brown) and these are only found in the IT. OMP+ Leydig-like cells are shown on the right at higher magnification. Scale bar = 50 µm for the tissue and 18 µm for the panel of individual cells.