Graph of Leydig and OMP+ cells representation in the IT of the rat testes. (A) Leydig cell population, as defined by immunolabeling by CR (CR+), represent the majority—79% of all cells (n=115) in the IT of rat testes. The CR-negative (CR–) cells represent the remaining 21%. (B) 81% of the Leydig cell population (n=91) was OMP+ (Double: CR+OMP+). No cells are labeled by OMP alone (OMP Single+) and CR+ only cells (CR Single+) are represented 19% of the Leydig cell population. Statistical significance between groups is indicated by asterisks. CR+ vs CR– (χ2 test, *p<0.001); double vs CR single+ (χ2 test, *p<0.001); double vs OMP single+ (F test, *p<0.001). Abbreviations: OMP, olfactory marker protein CR, calretinin; IT, interstitial tissue.