Figure 6.

Fluorescent photomicrographs showing compartment-specific expression of Golf and AC3 in the rat spermatozoa. (A) Expression of Golf (red) is seen on the acrosomal region (arrow) and EqS (crossed arrow) of the head, on the cp (yellow arrow), and along the tail in a discontinuous pattern. (B) Expression of AC3 (green) is seen in similar compartments: on the head (arrow, crossed arrow), on the cp (yellow arrow), and along the tail in a discontinuous pattern. Strong expression of Golf and AC3 is seen on the mp, gradually diminishing along the tail. DAPI (blue) stains spermatozoal nuclei. Scale bar = 10µm. Abbreviations: AC3, adenylyl cyclase 3; Golf, olfactory G protein; EqS, equatorial segment; cp, connecting piece; mp, middle piece; DAPI, 4′,6-diamidino-2-phenylindole dihydrochloride.