Figure 7.

Photomicrographs show compartment-specific expression of OMP in ejaculated human spermatozoa (control). (A) Phase-contrast image shows the structure of human spermatozoa, consisting of the head (bracket), cp (arrowhead), and tail. (B) Immunofluorescence shows expression of OMP (red) on the apical part of the acrosomal region (crossed arrows) and along the EqS of the head (arrows), on the cp (arrowheads), and along the tail, except for the mp. (C) DAPI (blue) shows spermatozoal nuclei, surrounded by a thin rim of cytoplasm. Acrosome is seen above nuclei, on the anterior part of the head (crossed arrows in B). Scale bar = 10 µm. Abbreviations: OMP, olfactory marker protein; cp, connecting piece; EqS, equatorial segment; mp, middle piece; DAPI, 4′,6-diamidino-2-phenylindole dihydrochloride.