Table 1.
Primary Antibodies and Their Specificity.
| Primary Antibody | Antibody Dilution |
Specificity | |||
|---|---|---|---|---|---|
| Rat |
Human |
||||
| OE | IT | Spermatozoa | |||
| OMP, goat antiserum (019-22291; Wako Pure Chemical Industries; Osaka, JP) | 1:1000IF1:25IP | 1:1000IF1:25IP | 1:800IF | 1:800IF | mOSNs |
| Golf, goat pAb (P-15) (sc-26763; Santa Cruz Biotechnology; Dallas, TX) | NA | NA | 1:250IF | NA | OSNs |
| AC3, rabbit pAb (C-20) (sc-588; Santa Cruz Biotechnology; Dallas, TX) | NA | NA | 1:250IF | NA | OSNs |
| OR6B2, rabbit pAb (NBP2-13711; Novus Biologicals; Littleton, CO) | NA | NA | NA | 1:100IF | OR6B2 |
| Calretinin, rabbit pAb (ab702; Abcam; Sydney, Australia) | NA | 1:400IF | NA | NA | Leydig cells |
Abbreviations: AC3, adenylyl cyclase 3; IT, interstitial tissue; IF, immunofluorescent histochemistry; IP, immunoperoxidase histochemistry; mOSNs, mature OSNs; OE, olfactory epithelium; OMP, olfactory marker protein; OR6B2, specific human olfactory receptor; OSNs, olfactory sensory neurons; NA, not applicable; pAb, polyclonal primary antibody.