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Summary of method development optimization.
| Drug | Mobile phase | pH | Diluent used | |
|---|---|---|---|---|
| Buffer | ACN | |||
| FAM and IND mixture | 93 | 7 | 6 | Diluent 1 |
| 93 | 7 | 5 | ||
| 50 | 50 | 5.5 | ||
| 60 | 40 | 5.5 & 6 | ||
| 40 | 60 | 5.5 & 5 | ||
|
| ||||
| Codrug | 40 | 60 | 5 | Diluent 1 |
|
| ||||
| PAR | 40 | 60 | 5 | Diluent 1 |
| Methanol | ||||
| ACN | ||||
|
| ||||
| FAM, codrug, and IND separately | 40 | 60 | 5 | ACN |
|
| ||||
| Mixture of all drugs | 40 | 60 | 5 | ACN |
