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. 2020 Jul 8;6(7):e04392. doi: 10.1016/j.heliyon.2020.e04392

Table 1.

Alpha-glucosidase inhibitory (AGI) activity, phytochemical analysis, antioxidant activity of ethanol crude extract and column purified ‘fraction-14’ of S. glauca leaf.

Sample AGI activity (IC50 μg/mL) Phytochemicals
Antioxidant activity (IC50 in μg/mL)
Total phenolics (mg GA/g) Total flavonoids (mg Q/g) DPPH Assay ABTS Assay
Ethanol crude extract 0.5 ± 0.04 414 ± 11 166 ± 29 12.5 ± 0.6 4.0 ± 0.5
Purified fraction-14 2.4 ± 0.40 178 ± 70 - 14.4 ± 0.1 7.6 ± 0.5
Ascorbic acid - - - 5.1 ± 0.1 3.9 ± 0.2
Acarbose 2450 ± 24 - - - -

Note: AGI activity and antioxidant activity are represented by IC50 values. Acarbose was used as a standard for AGI activity. Total phenolics are represented in mg GA per gram of extract. Total flavonoids are represented in mg Q per gram of extract. Ascorbic acid was used as standard for antioxidant activities. All the experiments are expressed as mean ± SD (n = 3). The values are statistically significant at p < 0.05.

Q: Quercetin, GA: Gallic acid, SD: Standard Deviation, “-“: Not determined.