Table 5.
Maximum velocity; Vmax and Michaelis-Menten constant; Km for different concentrations of column purified ‘fraction-14’.
| S.N. | Fraction-14 (μg/mL) | Vmax (μM/min.) | Km (μM) |
|---|---|---|---|
| 1 | 0 | 2.95 | 70 |
| 2 | 0.83 | 2.50 | 74 |
| 3 | 1.66 | 2.33 | 80 |
| 4 | 3.32 | 2.28 | 90 |
| 5 | 4.15 | 2.13 | 90 |
Note: The alpha glucosidase concentration was 0.02 units/mL. The substrate, PNPG concentration was 0.01, 0.02, 0.04, 0.08, 0.1, 0.2, 0.4 and 0.8 mM. The differences among the values are considered to be statistically significant at p < 0.05. PNPG: p-nitrophenyl-α-D-glucopyranoside.