Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 3;11:712. doi: 10.3389/fphys.2020.00712

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Cloos, Ghodsi, Stommen, Vanderroost, Dauguet, Pollet, D’Auria, Mignolet, Larondelle, Terrasi, Muccioli, Van Der Smissen and Tyteca.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

RBC-derived EVs are enriched in ceramide and dihydroceramide species at the end of storage but nearly depleted in sphingomyelin and phosphatidylcholine species. RBC-released EVs were isolated from total blood EVs by immunopurification and analyzed by LC-MS for their content in: (A,B) ceramide (cer) and dihydroceramide (dhcer) species; (C) SM d18:1 species; and (D–F) phosphatidylcholine (PC; presented in two graphs according to the length of their carbon chains) and lysoPC species. Each curve depicts one lipid species; circle, saturated lipid; triangle, lipid containing at least one MUFA; inverted triangle, lipid containing at least one PUFA; black to light gray and white colors, short to long carbon chains. Notice that most of the SM species (e.g., 16:1) and some PC species (e.g., 16:0_18:0) were at the limit of detection. Representative experiment out of 2. All data were expressed as % of the EVs stored for 1 week and are means ± SD of 4 replicates.