Skip to main content
. Author manuscript; available in PMC: 2021 May 28.
Published in final edited form as: Nanoscale. 2020 May 28;12(20):11070–11078. doi: 10.1039/d0nr01577f

Table 2.

Post-translational modifications to polyanionic C-terminal domains of αSyn and tubulin.

PTM CT residues
affected		 Effect on
residue
charge		 Increase in
residue
volume		 References Predicted
effects
α-Synuclein
Phosphorylation 125, 129, 133, 136 −2 54 Å3 41 Higher translocation probability, longer dwell time at low voltages
Nitration 125, 133, 136 0 53 Å3 Molecular volume from MW and density of NO2 liquid Minimal
Ubiquitination/Sumoylation 96 / 96, 102 0 / −5 (does not affect CTT properties) 11130 Å3 / 14890 Å3 42 Eliminates translocation, behaves like tubulin
Dopamination 125-129 0 150 Å3 33, 42 Smaller capture rate, longer dwell time at low voltages
Tubulin
Polyglutamylation (N residues) varies by isotype −(N + 1) N × 155 Å3 42 Longer dwell time, stronger dependence on voltage
Polyglycylation (N residues) varies by isotype −1 N × 66 Å3 42 Longer dwell time, same voltage dependence