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. 2020 Jun 18;8(2):317. doi: 10.3390/vaccines8020317

Figure 2.

Figure 2

Tumor-infiltrating T cells (TILs) were incubated with K-RasG12Dp53null tumor cells at ratio of 5:1 overnight followed by addition of almar blue for 4 h. TILs from mice treated with combined therapy were most effective at lysing K-RasG12Dp53null tumor cells; ** p < 0.01 in comparison to diluent control, * p < 0.05 compared to monotherapy. Results are representative of an independent experiment (A). The experiment was repeated twice (n = 4 mice/group). Flow cytometric analyses of single-cell suspensions of the tumor microenvironment (TME) following therapy showed enhancement of CD8 T cells expressing granzyme B (p < 0.05) compared to monotherapy. P98 is dumped cell population, P99 is dead cell population and P100 is CD8 T cell population. Results are representative of an independent experiment. The experiment was repeated twice (n = 4 mice/group) (B).