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(A,B) Tregs and NK cells from CCL21-DC lysate vaccine plus anti-PD-1 did not have a change in activity. Purified Tregs (1:5) from combined therapy did not alter the proliferation of anti-CD3/anti-CD28 (0.2/2 µg/mL) stimulated T cell proliferation. Almar blue (20 µL) was added for 4 h on day 3, and fluorescence was read at excitation/emission (530nm/595nm) in a Wallac Fluorescence reader.
