Figure 4.
Antibody response in SUB-vaccinated and control cattle. (A) B. indicus cattle. (B) crossbred cattle. Blood samples were collected before each vaccination (days 0, 30 and 60; violet arrows), at day 45 between second and third vaccinations and at the end of the experiment (days 180 or 195 for B. indicus and crossbred cattle, respectively). Serum IgG antibody titers were determined using an indirect antigen-specific ELISA. Antibody titers were expressed as the OD450 nm values and compared between vaccinated and control groups using a one-way ANOVA test (https://www.socscistatistics.com/tests/anova/default2.aspx) (* p < 0.05, ** p < 0.005; n = 4 biological replicates).