Figure 7.

Correlation analysis between anti-SUB antibody titers and vaccination effect on different tick developmental stages. (A) B. indicus cattle infested with R. appendiculatus. (B) crossbred cattle infested with R. appendiculatus. (C) crossbred cattle infested with R. decoloratus. (D) values (average + SD) of the parameters of the tick developmental stages used in the analysis (in red are shown the parameters with significant negative correlation with anti-SUB antibody titers and the threshold value line for observing significant differences). The correlation analyses were conducted between the values of the parameters of Rhipicephalus spp. tick life stages used for the calculation of vaccine E that showed significant differences with at least three of the SUB vaccine formulations (Table 1) and the anti-R. appendiculatus SUB antibody titers at day 60 before tick challenge. All animals in both vaccinated and control groups were included in the analysis. Data was analyzed using a Spearman’s Rho correlation analysis (p ≤ 0.05; n = 20, i.e., 4 animals for each of the 5 vaccine formulations including the control).