Figure 5.

Percentages (a) and geometric mean intensity (b) of Fluo3⁺ spermatozoa during in vitro capacitation and progesterone-induced acrosomal exocytosis (300 min) after previous storage of spermatozoa at 17 °C with different concentrations of seminal plasma (0%, 15%, and 30%) for 48 h or 72 h. Grey arrow indicates the time at which 10 μg/mL progesterone was added to induce acrosomal exocytosis (i.e., 240 min). Different letters mean significant (p < 0.05) differences between treatments at a given time point. Data are shown as mean ± SEM for seven independent experiments.