Figure 7.

Percentages of spermatozoa with high mitochondrial membrane potential (MMP; (a)) and their JC1_agg/JC1_mon ratios (b) during in vitro capacitation and progesterone-induced acrosomal exocytosis (300 min) after previous storage of spermatozoa at 17 °C with different concentrations of seminal plasma (0%, 15%, and 30%) for 48 h or 72 h. Grey arrow indicates the time at which 10 μg/mL progesterone was added to induce acrosomal exocytosis (i.e., 240 min). Different letters mean significant (p < 0.05) differences between treatments at a given time point. Data are shown as mean ± SEM for seven independent experiments.