Immunization against α-Gal-BSA increases fungal burden in turkeys and chickens. The levels of circulating anti-α-Gal IgY Abs to Galα1-3Gal, lung granuloma score and A. fumigatus CFU number and 28S levels in lungs, were quantified in turkeys (A–D) and chickens (E–H). Immunization against α-Gal-BSA increases the levels of anti-α-Gal IgY Abs to Galα1-3Gal and A. fumigatus 28S levels in turkeys (A,D) and chicken (E,H). Results shown are means and standard deviation values. Results were compared by unpaired non-parametric Mann–Whitney U test (* p < 0.0001, *** p < 0.0001; ns: not significant, 1 experiment with chicken, n = 10 and 2 experiments with turkeys, n = 20 and three technical replicates per sample in the ELISA (A,B) and qPCR (D,H) assays).