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. 2020 Jun 19;8(2):321. doi: 10.3390/vaccines8020321

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The central immune system is activated by simultaneous combination therapy. Splenocytes enumeration of CT26 tumor–bearing Balb/c mice. After 40 days, spleens were harvested from responders and a single-cell preparation was made for flow cytometry. CD45⁺, CD8⁺, and CD4⁺ cell populations were quantified. (a) Representative images of CD45⁺, CD4⁺, and CD8⁺ T-cell histograms in all six groups.(b) Total number of CD45⁺ cells in splenocytes.(c) CD4⁺ T-cells % in CD45⁺ cells in all six groups. (d) CD8⁺ T-cells % in CD45⁺ cells in all six groups.