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. 2020 Jun 25;9(6):512. doi: 10.3390/pathogens9060512

Table 4.

Details of primers used for amplifying mitochondrial and nuclear genes of recovered larvae.

Region Amplified Primer Sequence (5′-3′) Annealing T (°C) Product (bp) Ref
cox1 Fw COIintF TGATTGGTGGTTTTGGTAA 48 ~689 [44]
Rev COIintR ATAAGTACGAGTATCAATATC
12S rRNA Fw 12SF GTTCCAGAATAATCGGCT 50 ~502 [45]
Rev 12SR ATTGACGGATGRTTTGTACC
18S rRNA (SSU HVR-I) Fw RS5401 AAAGATTAAGCCATGCATG 50 ~919 [43]
Rev RS5402 CATTCTTGGCAAATGCTTTCG

Abbreviations: Fw = forward primer; Rev = reverse primer; T = temperature; Ref = reference.