Table 4.
Region Amplified | Primer | Sequence (5′-3′) | Annealing T (°C) | Product (bp) | Ref | |
---|---|---|---|---|---|---|
cox1 | Fw | COIintF | TGATTGGTGGTTTTGGTAA | 48 | ~689 | [44] |
Rev | COIintR | ATAAGTACGAGTATCAATATC | ||||
12S rRNA | Fw | 12SF | GTTCCAGAATAATCGGCT | 50 | ~502 | [45] |
Rev | 12SR | ATTGACGGATGRTTTGTACC | ||||
18S rRNA (SSU HVR-I) | Fw | RS5401 | AAAGATTAAGCCATGCATG | 50 | ~919 | [43] |
Rev | RS5402 | CATTCTTGGCAAATGCTTTCG |
Abbreviations: Fw = forward primer; Rev = reverse primer; T = temperature; Ref = reference.