Figure 6.

Analysis of IgG responses to OvMANE1 chimeric antigen in communities of different levels of endemicity. Purified OvMANE1_MBP chimeric antigen or MBP (control) was used to coat microtiter plates. Microtiter plates were blocked and later incubated with serum from O. volvulus serum (OVS) or Ivermectin treated serum (ITS) followed by incubation with goat anti-human IgG peroxidase conjugate. The microtiter plates were revealed using TMB, the optical density (OD) read at 450 nm and OD values were plotted against the different serum types. OVS, n = 52, ITS, n = 47. Mann-Whitney U test was used to compare the responses.