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. 2020 Jun 22;9(6):495. doi: 10.3390/pathogens9060495

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of humoral immune response to OvMANE1 chimeric antigen using sera pools from infected and non-infected individuals. Purified OvMANE1_MBP chimeric antigen was used to coat microtiter plates. Microtiter plates were blocked and incubated with serum pools from either OVS or ECS at a dilution from 1:250 to 1:32,000 followed by incubation with (A) mouse monoclonal anti-Human IgG4 (HRP) or (B) goat anti-human IgG peroxidase conjugate as the secondary antibody. The microtiter plates were revealed using TMB, the optical density (OD) was read at 450 nm and OD values were plotted against the different serum types. OVS = O. volvulus serum (n = pool of 10 infected serum samples), ECS = European control serum (n = pool of 3 control serum samples).

Analysis of humoral immune response to OvMANE1 chimeric antigen using sera pools from infected and non-infected individuals. Purified OvMANE1_MBP chimeric antigen was used to coat microtiter plates. Microtiter plates were blocked and incubated with serum pools from either OVS or ECS at a dilution from 1:250 to 1:32,000 followed by incubation with (A) mouse monoclonal anti-Human IgG4 (HRP) or (B) goat anti-human IgG peroxidase conjugate as the secondary antibody. The microtiter plates were revealed using TMB, the optical density (OD) was read at 450 nm and OD values were plotted against the different serum types. OVS = O. volvulus serum (n = pool of 10 infected serum samples), ECS = European control serum (n = pool of 3 control serum samples).