Table 2.
Primer and probe sequences used in this study.
| Primer | Sequence (5′→3′) | Position (nt) a | Virus | Region |
|---|---|---|---|---|
| Real-Time RT-PCR | ||||
| ZIKV835 | TTGGTCATGATACTGCTGATTGC | 835–857 | ZIKV | Matrix |
| ZIKV911c | CCTTCCACAAAGTCCCTATTGC | 911–890 | Envelope | |
| ZIKV860-FAM b | CGGCATACAGCATCAGGTGCATAGGAG | 860–886 | Envelope | |
| ZIKV1086 | CCGCTGCCCAACACAAG | 1086–1102 | ZIKV | Envelope |
| ZIKV1162c | CCACTAACGTTCTTTTGCAGACAT | 1162–1139 | Envelope | |
| ZIKV1107-FAM b | AGCCTACCTTGACAAGCAGTCAGACACTCAA | 1107–1137 | Envelope | |
| RT-PCR for Sequencing | ||||
| ZIKENVF | GCTGGDGCRGACACHGGRACT | 1643–1663 | ZIKV | Envelope |
| ZIKENVR | RTCYACYGCCATYTGGRCTG | 1989–2008 | Envelope | |
| D1 | TCAATATGCTGAAACGCGCGAGAAACCG | 132–159 | DENV | Capsid/prM |
| D2 | TTGCACCAACAGTCAATGTCTTCAGGTTC | 614–642 | Capsid/prM | |
a. The nucleotide numbering corresponds to that of the published ZIKV sequence [GenBank: AY632535.2] or DENV sequence [GenBank: DQ863638.1]. b. Probes are labeled with 5′ 6-FAM and 3′ TAMRA-N.