Table 5.
Comparison of results in autopsied fetuses according to high-resolution melting analysis (HRMA), lfb1 phylogeny and serology in dams.
HRMA rtPCR | lfb1 Sequencing | Serology of Dam | Necropsy | |||
---|---|---|---|---|---|---|
Fœtus ID | Sample ID | Tm | Species Group Attribution | Species Group Attribution | MAT | Group |
f050 | 15095924-1509927-159915 | 81.92 | interrogans | interrogans cluster 1b | 1/500 Australis | Icteric |
f033 | 14040522.1-1610225.2 | 81.93 | interrogans | interrogans cluster 1a | 1/1000 Icterohaemorrhagiae | Icteric |
f010 | 14048419.1-1610225.3 | 81.69 | interrogans | interrogans cluster 2 | 1/500 Australis | Icteric |
f032 | 14045546 | 81.71 | interrogans | interrogans cluster 1b | - | Icteric |
f044 | 14057925-1417554.1-1417819 | 81.72 | interrogans | interrogans cluster 1b | ND (1/100 Ballum 1/100 Grippothyphosa) | Icteric |
f028 | 15050216-1506066.2 | 82.96 | kirschneri | kirschneri cluster | 1/100 Grippotyphosa | Icteric |
f007 | 14047394.1-1610225.1 | 82.82 | kirschneri | - | 1/500 Grippotyphosa | Icteric |
f031 | 14044550 | 82.76 | kirschneri | kirschneri cluster | - | Icteric |