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(a) Absorption spectra of FAD (–––), FMN (–––) and ε-FAD (–––). Also shown are Ade (●) and ε-Ade (●) for comparison. The inset shows the region from 290 to 340 nm where the flavin molecules have a minimum in their absorbance, while the ε-Ade group has roughly 3 times the extinction at ca. 305 nm. (b) Photoreduction of ε-FAD→ε-FADH− does not reduce the ε-Ade molecule, as shown by the unchanged spectral band around 305 nm. FADH− is also shown for comparison.
