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. 2020 Jul 3;10:1072. doi: 10.3389/fonc.2020.01072

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Copyright © 2020 Zhou, Yang, Ao, Höti, Gabrielson, Chan, Zhang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Workflow for the proteomics detection in BAL specimen and subsequent validation study. The overall strategy of our study consists of following steps: (1) recovery of peptides from BAL samples, (2) capture of formerly N-linked glycopeptides using SPEG method, (3) Orbitrap Velos LC-MS/MS analysis, (4) identification and quantification of protein/peptide using Proteome Discoverer and SIEVE software, (5) analysis and comparison of the N-glycoprotein abundance in different BAL specimens, and (6) validation studies by using an ELISA assay.