Table 1.
Basal medium
| Reagent | volume | Final concentration |
|---|---|---|
| Advanced DMEM/F12 | 500 mL | |
| GlutaMAX (100x) | 5 mL | 1x |
| HEPES (1 M) | 5 mL | 10 mM |
| Antibiotic-Antimycotic (100x) | 5 mL | 1x |
| Gentamicin (50 mg/mL) | 50 μL | 5 μg/ml |
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