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. 2019 Nov 12;41(5):551–560. doi: 10.1093/carcin/bgz142

Figure 4.

Figure 4.

Bry positively regulates the transcription of SOX5. (A) Forty-four genes containing upstream, intergenic, intron, exon and promoter regions are listed. (B) Pie charts indicating the simulated distribution of ChIP-seq peaks. (C) The gene ontology analysis, as listed by P value, and the lengths represented the counts of related genes (P < 0.05). (D) Representative ChIP-seq peaks surrounding Bry target genes (SOX5) (−10*log10 (P value) >50). (E) Schematic showing the potential Bry-binding sites in the SOX5 promoter. (F) The recruitment of endogenous Bry to the promoter of SOX5 was detected using a ChIP-PCR assay. (G) SOX5 promoter activity from three respective fragments was evaluated using dual-luciferase reporter assays. (H–K) SOX5 promoter activity with mutated (containing deletion and point mutations) Bry-binding sites was evaluated using dual-luciferase reporter assays. (L) SOX5 promoter activity in Bry-knockdown cells compared with that in control cells (*P < 0.05, **P < 0.01, ***P < 0.001).