Fig. 3.

a Plotted transformation effiencies (CFU/μg) for pRS305, pRS315, pRS305 + CS-ARS1 + CEN6, and pRS315 + CS-ARS1 + CEN6. Statistical significance is noted by asterisks (***p-value = 0.0003; **p-value < 0.005; *p-value < 0.02; n.s., not significant). Smaller gray dots represent individual data points, larger black dot indicates sample average. Error bars represent standard deviation of the mean (n = 3 for all samples). b Tabulated data plotted in a. c Gel results for a colony PCR. Lanes are as follows: L, ladder; 1, pRS305 + CS-ARS1 + CEN6 plasmid; 2, pRS315 + CS-ARS1 + CEN6 plasmid; 3, wild type S. cerevisiae cells; 4, pRS305 transformed cells; 5, passaged pRS315 transformed cells, 6–8, triplicates of passaged pRS305 + CS-ARS1 + CEN6 transformed cells; 9–11, triplicates of passaged pRS315 + CS-ARS1 + CEN6 transformed cells