Fig. 4.

Fibroblast co-culture additionally lowers energy reserves and central carbon metabolites in Sdhb-silenced MTT cells compared to control. a Intracellular ATP levels were measured in four independent experiments with two replicates each. b, c LC-MS/MS-mediated measurements in MTT cells with fibroblast co-culture of intracellular metabolites of selected Krebs cycle acids (b) and amino acids (c). Asterisks indicate significance by one-way ANOVA/Bonferroni test (*p < 0.05), comparing Sdhb63 with and without co-culture; n.d. not detectable