Fig 4.

Summary of the deletion and alternative splicing in mdig mRNA from KO clones. A. Schematic of deletions and alternative splicing in KO clones. White bars represent exons and blank lines are introns. Red slash bars represent deletion or alternative spliced nucleotides. The red and green arrow indicate where the sgRNA-1 and sgRNA-3 are targeted respectively. B. Corresponding protein sequence. Mdig protein sequence is in black. The third exon protein sequence is shaded in pink. The nine and twenty-four nucleotides are corresponding to amino acids shown in black underlines or red underlines respectively. The amino acid substitute in KO cells are marked with bold. The sequence in red is the new alternative spliced form with new stop codon (*).