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. 2020 Jul 10;11:278. doi: 10.1186/s13287-020-01795-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Overexpression of ILK on BMSCs and its effect on proliferation and adhesion of BMSCs. a The mRNA expression of ILK was determined using qRT-PCR. *P < 0.05 versus BMSCs and Ad-null-BMSC group. b The mRNA expression of ILK downstream protein AKT, GSK-3β, and CDH1 was determined using qRT-PCR. *P < 0.05 versus BMSCs and Ad-null-BMSC group. c The protein expression of ILK and its downstream protein AKT, pAKT, GSK-3β, pGSK-3β, and CDH1 by western blot in the BMSCs. d A statistical chart of the relative optical density of ILK, AKT, pAKT, GSK-3β, pGSK-3β, and CDH1 in each group. *P < 0.05 versus BMSCs and Ad-null-BMSC group. e Adherent cells stained with Hoechst were observed using a Fluoview microscope in cell adhesion assay. f A statistical chart reveals the percentage of adherent cells by the total corrected fluorescence of the total cells in each group. *P < 0.05 versus BMSCs and Ad-null-BMSC group. g The proliferation level of cells in each group was determined using CCK-8 assay for 7 days. *P < 0.05 versus BMSCs and Ad-null-BMSC group