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. Author manuscript; available in PMC: 2020 Jul 10.
Published in final edited form as: J Bone Miner Res. 2019 Jun 7;34(6):979–995. doi: 10.1002/jbmr.3720

Figure 1: OmGFP66 cells form mineralized bone-like trabeculae over 7-28 days in culture.

Figure 1:

A) Time course in OmGFP66 cells showing expression of the Dmp1-mGFP reporter (green) and alizarin red fluorescence (red) to monitor calcium deposition. Note the initial formation of small bone-like structures containing mineral and Dmp1-mGFP-positive cells by day 7. These bone-like structures increase in number and size over 28 days, bar = 100μm. B) Lower power view showing the trabecular-like nature of the bone mineral, bar = 250μm. C, D) Quantitation of alizarin red stained area (C) and Dmp1-mGFP positive cell area (D) over 28 days (data are mean ± SEM, n= 4).