qPCR analysis of the timecourse of expression of A) osteocyte marker genes, E11/gp38, Dmp1, Phex, Mepe, Fgf23 and Sost by OmGFP66 cells and B) other markers, RankL, Opg, Cx43, Hif1α and Col1a1. Data were normalized to β-actin as a housekeeping control and are presented as the fold change compared to day 1 (mean ± SEM, n=3). Numbers in parentheses are the Ct values for the highest level of expression of each gene.