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. 2020 Jul 3;14:171. doi: 10.3389/fncel.2020.00171

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Copyright © 2020 Kegeles, Naumov, Karpulevich, Volchkov and Baranov.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Retinal differentiation. (A) Experimental outline: the organoids were imaged on day 5 using bright-field and on day 9 using fluorescent microscopy. Fluorescent images were used to assign true labels and bright-field ones for feeding neural network. This figure was created with BioRender.com. (B) Confocal image of retinal organoid on day 9 of retinal differentiation. Staining was performed for early retina-specific markers: Rx and Pax6. (C) Representative organoids from retinal and non-retinal classes. Different patterns in fluorescent images reflect the difference in bright-field ones.