Knockdown of miR-204-5p did not significantly influence the development of modest hypertension in SS.13BN26 rats fed a high-salt diet. (A) miR-204-5p abundance in the renal cortex in SS rats compared with protected SS.13BN26 rats fed a 4% NaCl diet for 3 or 7 days (n=6/group). *P<0.05, two-way ANOVA followed by Holm–Šidák test. (B) miR-204-5p abundance in the kidneys of SS.13BN26 rats treated with anti–miR-204-5p. For renal cortex, n=12 in scrambled anti-miR group, n=9 in anti–miR-204-5p group. For renal outer medulla, n=3 per group. *P<0.05 compared with scrambled anti-miR group, t test. (C–E) Mean arterial BP (MAP), systolic BP (SBP), and diastolic BP (DBP) in SS.13BN26 rats fed a 4% NaCl diet. Scrambled anti-miR or anti–miR-204-5p was injected intraperitoneally at 2 mg/kg body wt on the days indicated by the arrows. n=12 in scrambled anti-miR group, n=9 in anti–miR-204-5p group.