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. 2020 Jun 2;31(7):1539–1554. doi: 10.1681/ASN.2019101100

Figure 8.

Figure 8.

Treatment with anti–miR-204-5p exacerbates renal histologic injury and upregulates SHP2 in diabetic db/db mice. (A) Representative trichrome staining of kidney sections showing interstitial fibrosis (blue) in renal cortex and medulla in db/db mice after 12 weeks of treatment with anti–miR-204-5p or scrambled anti-miR. Scale bar, 100 µm; original magnification, ×200. (B) Semiquantitative analysis of interstitial fibrosis shown in (A). (C) Representative periodic acid–Schiff staining of kidney sections showing mesangial expansion and extracellular matrix deposition and semiquantitative analysis in db/db mice after 12 weeks of treatment with anti–miR-204-5p or scrambled anti-miR. Scale bar, 50 µm; original magnification, ×400. (D) Cytokine or chemokine mRNA abundance, (E) SHP2 mRNA and protein abundance, (F) STAT3, and (G) Src activation measured by ELISA, and (H) SP1 and IL-6 receptor mRNA abundance in the renal cortex of db/db mice after 12 weeks of treatment with anti–miR-204-5p or scrambled anti-miR. n=6 for each group. *P<0.05, versus db/m group and #P<0.05, versus scrambled anti-miR treatment, one-way ANOVA followed by Holm–Šidák test.