Figure 5.

RMIC ETA KO regulates inner medullary ENaC, NOS and COX protein expression. The figure shows western blot analysis of ENaC, NOS-1 and -3, and COX-1 and -2 in control and ETA KO mice fed normal and high Na⁺ diets. (A) Representative Western blots for ENaC isoforms (left) and COX and NOS isoforms (right) in inner medulla from ETA KO and control mice fed a normal and high Na⁺ diet. (C) Densitometry of these proteins normalized to normal salt (top) or high-salt (bottom) controls (n=7–8 each group). (B) Western blot and (D) densitometry for COX and NOS isoforms in IMCDs from control and ETA KO mice fed a high-salt diet (no COX2 was detected in IMCDs) (n=6 each group). Numbers by Western blots refer to molecular size in kDa. Circles indicate males and squares indicate females in dot plots. *P<0.05 KO versus control.