Figure 4.

The renal protective effect of Sult1e1 ablation is estrogen- and androgen-independent. (A) Mice are the same as described in Figure 3A. Shown are the serum levels of E₂. (B) Scheme of castration or ovariectomy followed by renal ischemic AKI. (C and D) Intact or castrated (CasKO) Sult1e1 KO males were subjected to sham surgery or AKI surgery. Shown are (C) serum levels of creatinine and BUN, and (D) renal histology as evaluated by PAS and TUNEL staining with their quantifications shown on the right with asterisks indicating tubular damage). (E and F) Intact or ovariectomized (OvxKO) Sult1e1 KO females were subjected to sham surgery or AKI surgery. Shown are (E) serum levels of creatinine and BUN, and (F) renal histology as evaluated by PAS and TUNEL staining with their quantifications shown on the right (with asterisks indicating tubular damage). (G) Uterine weights as percentages of the body weight in female mice subjected sham surgery or ovariectomy. n=4–6 for each group. Scale bars are 50 µm. Results are presented as the mean±SD.