Figure 6.

The renal protective effect of Sult1e1 ablation is associated with renal regulation of vitamin D-metabolizing and cell cycle genes. (A) Renal mRNA expression of Cyp24a1, Ccnd1, and Fgg in WT and Sult1e1 KO mice subjected to 30 minutes of ischemic AKI. (B and C) The renal expression of (B) Cyp24a1 and (C) Cyclin D1 in WT, Sult1e1 KO, and KOLE mice subjected to 30 minutes of ischemic AKI was shown by immunofluorescence. (D) The renal expression of Cyclin D1 protein in WT and Sult1e1 KO mice subjected to 30 minutes of ischemic AKI is shown by western blotting with the signal quantifications labeled. (E) Immunostaining of Ki67 in the same groups, with arrowheads indicating positive staining. (F) 293T cells were transfected with a VDR reporter gene tk-VDRE in the presence of the cotransfection of the VDR and SULT1E1 plasmids alone or in combination. Transfected cells were treated with vehicle (ethanol) or calcitriol (10 µM) for 24 hours before cell lysis and luciferase assay. n=3–8 per group. Scale bars are 50 µm. Results are presented as the mean±SD. **P<0.01; ***P<0.001; ****P<0.0001; compared with (A) WT AKI or (F) the comparisons are labeled. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.