Figure 2.

Luteolin has a favorable toxicity profile and improves hatching rates and deformity rates in ctns^−/− zebrafish larvae. (A and B) Survival rates in wild-type and ctns^−/− embryos and larvae after 0, 100, or 500 µM luteolin. Data are reported as mean±SEM (n=3 independent experiments). (C and D) Hatching rates in surviving wild-type and ctns^−/− embryos evaluated at 48, 72, and 96 hpf with 0, 100, or 500 µM of luteolin. (E and F) Deformity rates in wild-type and ctns^−/− larvae after 0, 100, or 500 µM luteolin. The total numbers of embryos evaluated for survival, hatching, and deformity rates were 97 embryos per group of wild-type and 350 embryos per group of ctns^−/− zebrafish. Luteolin was administered at 48 hpf in all experiments dissolved in the swimming water with the specified concentrations. **P<0.01 and ***P<0.001 using Fisher test.