Figure 5.

Luteolin treatment reduces oxidative stress in cystinotic cells. (A) Ctrl and CTNS^−/− ciPTCs were left untreated or pretreated with 50 μM luteolin for 24 hours. Levels of cellular ROS were then analyzed both at basal conditions and after 2 hours exposure to 100 μM tert-butyl- hydroperoxide (TBHP) by CellROX staining and flow cytometry. Diagram shows CellROX mean intensities normalized to those of untreated Ctrl. Data are presented as mean±SEM from nine independent experiments. t test *P<0.05; **P<0.01; and ***P<0.001 . (B) Representative images and quantification of CellROX (green) staining in wild-type and Ctns^−/− mPTCs under basal condition or treatment with 50 μM luteolin for 24 hours. Scale bar is 10 μm. CellROX fluorescence intensities per cell are reported; means and SEM are shown in red (n>100 cells, one-way ANOVA followed by Bonferroni post hoc test, ***P<0.001 and NS not statistically significant ).