Figure 7.

Luteolin restores impaired morphology of lysosomes in the pronephros of ctns^−/− larvae. Representative block-face scanning electron microscopy images of the proximal tubule of 5 dpf wild-type and ctns^−/− larvae after treatment without or with luteolin (100 μM for 72 hours). Arrowheads indicate lysosomes >1.5 µm diameter. Arrows indicate lysosomes <1.5 µm diameter. Scale bar is 5 μm. Diameter of lysosomes was quantified in five randomly selected fields (n=80 lysosomes from four tubules of two larvae for each experimental condition). Mann–Whitney U test ***P<0.001 and NS not statistically significant .