Figure 6.
Importance of T-cadherin in hMSCs for Adiponectin-Induced Exosome Production and Cardioprotection In Vivo
(A) Experimental design for intravenous injection of hMSCs in the transverse aortic constriction (TAC) model. hMSCs were transfected with control or T-cad (CDH13) RNAi and injected at a concentration of 5.0 × 105 cells per body via the tail vein the day after transfection. Injections were repeated six times at 2- to 3-day intervals within a period of 2 weeks. Echocardiography was performed at day 14. (B) Relative expression of T-cadherin in hMSCs after RNAi transfection at an indicated time points (n = 2). (C) Representative immunoblots. Serum exosomes were subjected to western blot analysis with the indicated antibodies to exosome markers. (D) Serum exosome marker levels from western blots (n = 3–4). (E) Heart weight per tibia length ratio (n = 6–8). (F) Representative images of echocardiography. (G) Ejection fraction (EF) and fractional shortening (FS) at 2 weeks after TAC or sham surgery (n = 6–8). Data are mean ± SEM. All subjects in this figure were tested in two separate trials. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 versus sham. †p < 0.05; ††p < 0.01; †††p < 0.001 between groups, by one-way analysis of variance with post hoc Tukey’s multiple comparisons.