Skip to main content
. Author manuscript; available in PMC: 2020 Jul 28.
Published in final edited form as: Cold Spring Harb Symp Quant Biol. 2020 Jan 3;84:95–104. doi: 10.1101/sqb.2019.84.039206

Figure 3. BIRC3 has 3′UTR-independent as well as 3′UTR-dependent interaction partners.

Figure 3.

(A) Western blot validation of endogenous BIRC3 interactors. 3′UTR-independent (orange bar) and 3′UTR-dependent (red bar) BIRC3 protein interactors identified by GFP co-immunoprecipitation in HEK293T cells after transfection of constructs containing the coding region of BIRC3 fused to GFP and the short 3′UTR (SU) or the long 3′UTR (LU). 1% of input was loaded. (B) 3′UTR-independent BIRC3 functions are mediated by 3′UTR-independent protein interactors (brown), whereas 3′UTR-dependent BIRC3 functions are mediated by 3′UTR-dependent interactors (blue and purple colors). 3′UTR-dependent functions can only be accomplished by BIRC3-LU. Reprinted from Lee & Mayr, 2019.