Table 3. . HTS results summary.

Bacteria spike-in: concentrations	Positive specific reads‡	Total reads	Positive reads per million
YP: 105/ml	22,212	8,396,771	2645
YP: 104/ml	5252	17,471,984	301
YP: 103/ml	655	10,344,988	63
BA: 105/ml	3447	20,799,540	166
BA: 104/ml	519	15,747,542	33
BA: 103/ml	–	11,741,665	–
FT: 105/ml†	2427	4,072,348	596
FT: 104/ml†	215	5,367,217	40
FT: 103/ml†	–	5,720,326	–

^†= samples that were ran in a multiplex mode.

^‡The strains that were identified by PathoScope are as follows: Yersinia pestis CO92 for YP, Bacillus anthracis str. CDC 684 for BA and Francisella tularensis subsp. holarctica LVS for FT.

Spiked-in: YP: Y. pestis strain EV76. BA: B. anthracis strain Vollum ΔpXO1ΔpXO2 (ATCC 14578). FT: F. tularensis LVS.